| Khoom npe | PNC-27 Kev tshawb fawb Peptide/HDM2-binding Peptide PNC-27 |
| Amino Acid Sequence | PLSSQETFSDLWKLLKKWKKMRRNQFW VKVQRG (p53 residues 12-26 + penetratin) |
| NCI Thesaurus Code | C2934281 |
| MeSH Cim ID | C549751 |
| Cia | Khaws PNC-27 hmoov ntawm -20℃; tom qab reconstitution, aliquot thiab khov tam sim ntawd - tsis txhob rov qab khov-thaw mus |
Khoom Description
Dab tsi yog PNC-27 Peptide?
PNC-27 (lub npe tag nrho: HDM2-binding Peptide PNC-27) yog ib qho kev sim tshuaj tiv thaiv kab mob peptide uas tau lees paub los ntawm National Cancer Institute (NCI) thiab National Institutes of Health (NIH).
Daim ntawv thov thiab kev tshawb fawb siv ntawm PNC-27 Peptide
Raws li kev txheeb xyuas cov kev tshawb fawb los ntawm National Institutes of Health (NIH) thiab National Cancer Institute (NCI), ntawm no yog cov lus qhia dav dav ntawm PNC-27 daim ntawv thov peptide.
1. Daim Ntawv Thov Tseem Ceeb: Kev Tshawb Fawb Kev Tiv Thaiv Kab Mob
PNC-27 daim ntawv thov thawj thiab feem ntau tau kawm yog raws li kev sim tshuaj tiv thaiv kab mob qog noj ntshav uas ua rau cov qog cell necrosis los ntawm cov txheej txheem tshiab hu ua "poptosis" (peptide-induced transmembrane pore formation
Mechanism-based Application
Cov peptide ua haujlwm los ntawm kev khi rau HDM-2 cov protein qhia tawm ntawm cov qog nqaij hlav qog nqaij hlav-ib cov protein uas feem ntau tsis tuaj ntawm cov cell membranes. Qhov kev sib txuas no ua rau:
| Kauj ruam | Kev tshwm sim | Qhov tshwm sim |
|---|---|---|
| 1 | PNC-27 daim ntawv 1: 1 complexes nrog membrane-bound HDM-2 | Temperature-independent khi |
| 2 | Complexes dimerize los ua transmembrane pores | Siab kub-nyob |
| 3 | Cov ntsiab lus ntawm tes sai sai tawm | Tumor cell necrosis (poptosis) |
2. Hom mob qog noj ntshav kuaj
PNC-27 tau qhia txog kev ua cytotoxic tawm tsam ntau hom qog nqaij hlav hauv tib neeg:
Cov qog nqaij hlav
| Hom mob qog noj ntshav | Cov kab mob tau kuaj | Nrhiav qhov tseem ceeb |
|---|---|---|
| Pancreatic Cancer | TUC-3, MIA-PaCa-2 | Ua kom tiav cell tua; ua tiav hauv vivo qog eradication |
| Cervical Cancer | HTB-35 (SiHa) | IC 50=12.4 μM |
| Mob cancer mis | Ntau kab | Ceev tag nrho necrosis (hauv 1 teev) |
| Ovarian Cancer | Ntev-tsim kab | Siv tau rau cov tshuaj tua kab mob-resistant |
| Colon Cancer | Ntau kab | Cytotoxic |
| Non-Small Cell Lung Carcinoma | Ntau kab | Siv tau |
| Melanoma | A2058 | Siv tau zoo hauv vitro thiab hauv vivo |
| Osteogenic Sarcoma | SAOS 2 | Siv tau (p53-null) |
| Angiosarcoma | Ntau kab | Siv tau |
3.Diagnostic Application: HDM-2 Kev Ntsuas
Cov peptide qhov tshwj xeeb khi rau HDM-2 muaj peev xwm siv hauv kev kuaj xyuas cov membrane-bound HDM-2 qhia hauv:
1) Cervical cancer hlwb (ruaj khov nyob rau hauv ob lub cell kab lis kev cai thiab cawv preservative daws) -
2) Cov qog loj hlob sai
3) Metastatic mob qog noj ntshav
4. Kev Tshawb Fawb Kev Siv: Mechanism Visualization
PNC-27 tau siv los ua ib qho cuab yeej los kawm txog kev tsim cov kab mob transmembrane pore siv high-resolution scanning immuno-electron microscopy, tso cai rau pom qhov pores hauv cov qog nqaij hlav cancer.
Cov txiaj ntsig ntawm PNC-27 Peptide
Raws li kev txheeb xyuas cov kev tshawb fawb los ntawm National Institutes of Health (NIH) thiab National Cancer Institute (NCI), ntawm no yog cov lus qhia dav dav ntawm PNC-27 peptide cov txiaj ntsig tseem ceeb hauv kev tshawb fawb txog qog noj ntshav.
1.High Selectivity: tua kab mob qog noj ntshav, Spares li qub
Qhov txiaj ntsig tseem ceeb tshaj plaws ntawm PNC-27 yog nwszoo kawg li selectivityrau cov qog nqaij hlav cancer dua li cov hlwb qub.
| Hom Cell | rhiab heev rau PNC-27 | Yog vim li cas |
| Cov qog nqaij hlav cancer (khoom thiab hematopoietic) | Siab | Siab HDM-2 qhia ntawm daim nyias nyias |
| Lub cev nqaij daim tawv (PCS-480) | Tsis muaj | Tsis muaj daim nyias nyias HDM-2 |
| Ib txwm pancreatic acinar cells (BMRPA1) | Tsis muaj | Tsis muaj daim nyias nyias HDM-2 |
| Ib txwm hematopoietic qia hlwb | Tsis muaj | Tsis muaj daim nyias nyias HDM-2 |
| Tib neeg fibroblasts, keratinocytes, HUVEC | Tsis muaj | Tsis muaj daim nyias nyias HDM-2 |
Kev tshawb nrhiav tseem ceeb los ntawm 2025 txoj kev tshawb nrhiav qog nqaij hlav qog noj ntshav: PNC-27 yog cytotoxic rau tib neeg lub tsev menyuam qog noj ntshav (IC50=12.4 μM) tab sis tsis muaj kev cuam tshuam rau lub tsev me nyuam li qub -2-5.
2024 AML kev tshawb fawb: PNC-27 xaiv lub hom phiaj ntawm daim nyias nyias HDM2 (mHDM2) qhia ntawm AML blasts thiab leukemia qia hlwb (LSCs) tab sis spares ib txwm hematopoietic qia hlwb (HSCs).
2.Effective Against Chemotherapy-Resistant Cancers
Ib qho txiaj ntsig tseem ceeb ntawm PNC-27 yog nws lub peev xwm los tua cov kab mob khees xaws-resistant. Cov tshuaj tiv thaiv kab mob qog noj ntshav tsis yog nyob ntawm cov txheej txheem intracellular uas feem ntau ua rau cov tshuaj tiv thaiv
Tsis nyob ntawm:
1) Tej teeb liab transduction txoj kev
2) DNA-kho cov txheej txheem
3) DNA methylation txheej xwm
4) Ntau hom tshuaj tiv thaiv (MDR) cov khoom lag luam
Cov pov thawj:
1) PNC-27 yog cytotoxic rau OVCAR-3 hlwb uas muaj ntau yam tshuaj tiv thaiv
2) Ua tau zoo ntawm kev siv tshuaj khomob ntev-tiv taus tib neeg lub zes qe menyuam kab mob qog noj ntshav
3) tua thawj cov qog nqaij hlav zes qe menyuam tshiab cais tawm ntawm cov neeg mob
Tseem ceeb Disclaimer
Tag nrho cov txiaj ntsig tau piav qhia yog los ntawm kev tshawb fawb txog kev tshawb fawb (hauv vitro thiab tsiaj qauv). PNC-27 Tsis yog FDA pom zoo kho mob. Cov ntaub ntawv no tsuas yog siv rau kev kawm thiab kev tshawb fawb.
Cov lus teb rau cov neeg siv khoom
Hu rau PAGE
1.Nrhiav txoj kev:
Email: alice@xaheshun.com
whatspp: +8618509260106
Online instant messaging
2. Hu rau cov ntaub ntawv:
Lub npe: Alice
Xov tooj: +8618509260106
Email: alice@xaheshun.com
FAQ
Q: Puas yog PNC-27 ua haujlwm ntawm p53-tsis muaj qog noj ntshav?
A: Yog, qhov no yog qhov txiaj ntsig tseem ceeb.
Q: Qib purity ntawm koj PNC-27 peptide yog dab tsi?
A: Peb PNC-27 peptide yog synthesized nrog ib tug guaranteed purityNtau dua lossis sib npaug98%
Q: Qhov no puas yog PNC-27 peptide npaj rau tib neeg noj lossis siv kho mob?
A: Tsis yog. Peb PNC-27 peptide yog muab nruj me ntsis rauKev tshawb fawb hauv chav kuaj siv thiab hauv vitro / hauv vivo kev sim lub hom phiaj nkaus xwb. Nws tsis pom zoo rau tib neeg noj, kev sim tshuaj, kev kuaj mob, lossis kev siv tshuaj kho mob. Txhua tus neeg yuav khoom yuav tsum yog cov kws tshawb fawb tsim nyog lossis cov tsev kawm ntawv tso cai.
Q: Cov kuab tshuaj dab tsi raug pom zoo rau kev rov tsim dua PNC-27 peptide?
A: NC-27 yog soluble heev hauv cov dej tsis muaj menyuam (H2O) lossis cov dej tsis zoo xws li PBS ntawm qhov siab txog lossis ntau dua 100 mg / mL. Rau kev ua haujlwm ntawm tes, peb pom zoo kom rov tsim kho cov tshuaj hauv cov dej tsis huv, lim nws los ntawm 0.22μm lim, thiab tom qab ntawd ua qhov kawg dilutions hauv qhov nruab nrab qhov ntsuas tsim nyog.
Q: Qhov kev tshawb fawb tam sim no ntawm PNC-27 yog dab tsi?
A: Raws li covNational Cancer Institute (NCI), PNC-27 yog muab faib ua a"biologically active tshuaj"hauv preclinical/translational research stage
Cim npe nrov: hdm2-binding peptide pnc-27, Tuam Tshoj hdm2-binding peptide pnc-27 manufacturers, lwm tus neeg, Hoobkas
